Additional file 5: of Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice

Figure S5. Inhibition of kinase activity of SAPK10 by OsPP2C50 or OsPP2C53 In vitro kinase assay were performed with OsPP2C50 or OsPP2C53. Each protein were used indicated quantity (below) and phosphorylated with SAPK10 in reaction buffer (60 mM NaCl, 20 mM Tris-Cl, pH 7.4, 5 mM MgCl2, 10 μM ATP, and 10 μCi γ-32P-ATP) at 30 °C for 30 min. (A) Detected phosphor image, (B) Polyacrylamide gel stained with Coomassie brilliant blue R250. (TIF 2460 kb)