Additional file 3: of Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus

(a) Metaphase counts of DLD-1 cells consistently show pseudo-diploid chromosome numbers of 44–46. Scale bar ~ 10 μm. (b) Immunostaining for Lamin B2 (red) upon Lamin A/C and Emerin co-depletion in DLD-1 cells. Scale bar ~ 10 μm. (c) Representative western blot showing Actin levels in whole cell extract of DLD-1 cells with single and combined depletion of Lamin A/C-Emerin. (d) Densitometric quantification of band intensities for Actin in whole cell extracts from cells with single and combined depletion of Lamin A/C-Emerin. Error bar: SEM, compiled data from N = 3 independent biological replicates, *p