Re-screening the candidate genes identified in previous Wg and Hh screens using the DRSC-v dsRNAs

Wg assay: 148 of 204 (73%) dsRNAs screened had reproducible effects on the Wg responsive reporter activity (a); 58% of the DRSC1.0 dsRNAs that were predicted to have OTEs repeated in the validation screen (b); while 84% of DRSC1.0 dsRNAs that were predicted to have ≥5 OTs could be reproduced with DRSC-v dsRNAs (c). Hh assay: 179 of 351 (51%) validation dsRNAs had reproducible effects on the Hh responsive reporter activity (d); 24% of the DRSC1.0 dsRNAs that were predicted to have OTEs repeated in the validation screen (e); while 64% of DRSC1.0 dsRNAs that were predicted to have ≥5 OTs could be reproduced with DRSC-v dsRNAs (f).Copyright information:Taken from "A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in "http://genomebiology.com/2007/8/9/R203Genome Biology 2007;8(9):R203-R203.Published online 28 Sep 2007PMCID:PMC2375041.