Additional file 4: of Differential expression analysis of Trichoderma virens RNA reveals a dynamic transcriptome during colonization of Zea mays roots

Figure S1. RT-qPCR validation of RNA-Seq based differential expression analysis. 9 DEGs representing a range of log2fold changes and expression in the Recognition vs Colonization datasets were chosen for validation with RT-qPCR (total of 18 data-points). Points are colored by DEG ID # to highlight RT-qPCR still captures the direction of the log2fold change when DEGs were oppositely regulated between Recognition and Colonization. The direction of the log2fold change was in agreement between the two methodologies, though the magnitude of said change was different. This is explicable by the different kits used for cDNA conversion and that the RT-qPCR samples were extracted from tissue held at -80ÍŚC for 12â months whereas samples used in the RNA-Seq analysis were extracted within one month. Pearson correlation was 0.75. (PPTX 38 kb)