Additional file 9: of Circular RNA AKT3 upregulates PIK3R1 to enhance cisplatin resistance in gastric cancer via miR-198 suppression

Figure S7. a The expression levels of PIK3R1 were analyzed using RT-qPCR. SGC7901CDDP cells were cotransfected with inhibitors alone or the indicated vectors. b The IC50 of circAKT3 was analyzed by the CCK8 assay. SGC7901CDDP cells were transfected with inhibitor alone or cotransfected with the indicated vectors upon CDDP exposure (6 μM) for 48 h. c The apoptosis rates of SGC7901CDDP cells transfected with inhibitor alone or cotransfected with the indicated vectors upon CDDP exposure (6 μM) for 48 h. d Immunofluorescence staining of γH2AX foci in SGC7901CDDP cells transfected with inhibitor alone or cotransfected with the indicated vectors at 2 h after CDDP treatment (6 μM). Scale bars, 10 μm. e Percentage of cells containing >10 γH2AX foci in SGC7901CDDP cells transfected with inhibitor alone or cotransfected with the indicated vectors at 0 to 8 after CDDP treatment (6 μM) removal. f The expression levels of PIK3R1, apoptosis markers, γH2AX, BRCA1 and PI3K/AKT signaling molecules were determined using western blotting in SGC7901CDDP cells transfected with inhibitor alone or cotransfected with the indicated vectors after CDDP treatment (6 μM). The results are presented as the mean ± SEM. *P