Additional file 6: of Circular RNA AKT3 upregulates PIK3R1 to enhance cisplatin resistance in gastric cancer via miR-198 suppression
Figure S4. a Predicted secondary structure of circAKT3 using the Vienna RNA package. b The expression of miR-198 was analyzed using RT-qPCR in tissues of cohort 1. c, d, e The relative luciferase activities were analyzed in 293T cells cotransfected with miR-198 mimics or miR-NC and luciferase report...
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| creator | Huang, Xiaoxu Li, Zheng Zhang, Qiang Wang, Weizhi Li, Bowen Wang, Lu Xu, Zhipeng Zeng, Ailiang Zhang, Xing Zhang, Xuan He, Zhongyuan Li, Qiang Sun, Guangli Wang, Sen Li, Qing Wang, Linjun Zhang, Lu Xu, Hao Xu, Zekuan |
| description | Figure S4. a Predicted secondary structure of circAKT3 using the Vienna RNA package. b The expression of miR-198 was analyzed using RT-qPCR in tissues of cohort 1. c, d, e The relative luciferase activities were analyzed in 293T cells cotransfected with miR-198 mimics or miR-NC and luciferase reporter vectors containing the WT or Mut 3’UTR of CHRM3 (C), HIPK2 (D), and MAFB (E). f & g The relative luciferase activities were analyzed in SGC7901CDDP(f) and BGC823CDDP(g) cells cotransfected with miR-198 mimics or miR-NC and luciferase reporter vectors PIK3R1 3’UTR (WT) or PIK3R1 3’UTR (Mut). h & i The expression levels of PIK3R1 (h) and miR-198 (i) in SGC7901CDDP and BGC823CDDP cells after transfection of PIK3R1 plasmids or miR-198 mimics were detected by RT-qPCR. j The IC50 of miR-198 was analyzed by the CCK8 assay. BGC823CDDP cells were transfected with miR-198 mimic alone or cotransfected with the indicated vectors upon CDDP exposure (15 μM) for 48 h. The results are presented as the mean ± SEM. *P |
| doi_str_mv | 10.6084/m9.figshare.7929242 |
| format | Image |
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BGC823CDDP cells were transfected with miR-198 mimic alone or cotransfected with the indicated vectors upon CDDP exposure (15 μM) for 48 h. The results are presented as the mean ± SEM. *P<0.05, **P<0.01, ***P<0.001. (TIF 1650 kb)</description><identifier>DOI: 10.6084/m9.figshare.7929242</identifier><language>eng</language><publisher>figshare</publisher><subject>Biochemistry ; Biological Sciences not elsewhere classified ; Biophysics ; Cancer ; Chemical Sciences not elsewhere classified ; FOS: Biological sciences ; FOS: Clinical medicine ; Genetics ; Hematology ; Immunology ; Marine Biology ; Molecular Biology ; Virology</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1892</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.7929242$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Huang, Xiaoxu</creatorcontrib><creatorcontrib>Li, Zheng</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Wang, Weizhi</creatorcontrib><creatorcontrib>Li, Bowen</creatorcontrib><creatorcontrib>Wang, Lu</creatorcontrib><creatorcontrib>Xu, Zhipeng</creatorcontrib><creatorcontrib>Zeng, Ailiang</creatorcontrib><creatorcontrib>Zhang, Xing</creatorcontrib><creatorcontrib>Zhang, Xuan</creatorcontrib><creatorcontrib>He, Zhongyuan</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Sun, Guangli</creatorcontrib><creatorcontrib>Wang, Sen</creatorcontrib><creatorcontrib>Li, Qing</creatorcontrib><creatorcontrib>Wang, Linjun</creatorcontrib><creatorcontrib>Zhang, Lu</creatorcontrib><creatorcontrib>Xu, Hao</creatorcontrib><creatorcontrib>Xu, Zekuan</creatorcontrib><title>Additional file 6: of Circular RNA AKT3 upregulates PIK3R1 to enhance cisplatin resistance in gastric cancer via miR-198 suppression</title><description>Figure S4. a Predicted secondary structure of circAKT3 using the Vienna RNA package. b The expression of miR-198 was analyzed using RT-qPCR in tissues of cohort 1. c, d, e The relative luciferase activities were analyzed in 293T cells cotransfected with miR-198 mimics or miR-NC and luciferase reporter vectors containing the WT or Mut 3’UTR of CHRM3 (C), HIPK2 (D), and MAFB (E). f & g The relative luciferase activities were analyzed in SGC7901CDDP(f) and BGC823CDDP(g) cells cotransfected with miR-198 mimics or miR-NC and luciferase reporter vectors PIK3R1 3’UTR (WT) or PIK3R1 3’UTR (Mut). h & i The expression levels of PIK3R1 (h) and miR-198 (i) in SGC7901CDDP and BGC823CDDP cells after transfection of PIK3R1 plasmids or miR-198 mimics were detected by RT-qPCR. j The IC50 of miR-198 was analyzed by the CCK8 assay. BGC823CDDP cells were transfected with miR-198 mimic alone or cotransfected with the indicated vectors upon CDDP exposure (15 μM) for 48 h. The results are presented as the mean ± SEM. *P<0.05, **P<0.01, ***P<0.001. (TIF 1650 kb)</description><subject>Biochemistry</subject><subject>Biological Sciences not elsewhere classified</subject><subject>Biophysics</subject><subject>Cancer</subject><subject>Chemical Sciences not elsewhere classified</subject><subject>FOS: Biological sciences</subject><subject>FOS: Clinical medicine</subject><subject>Genetics</subject><subject>Hematology</subject><subject>Immunology</subject><subject>Marine Biology</subject><subject>Molecular Biology</subject><subject>Virology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2019</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdj8tqw0AMRWeTRWn6Bd3oB-L4EdJMdia0pARKMdkPYiw7Ar-QxoXu8-GdlPQHuhL3CF1xjHnO0mSb7jbr3iYNt3pBoeTF5jbf5A_mWtY1Bx4H7KDhjmC7h7GBA4ufOxSoPkooT-cC5kmojSiQwuf7qagyCCPQcMHBE3jWKe54ACFlDb8wphY1CHvwNyDwxQg9V6vM7kDnKVaqxt9Ls2iwU3q6z0dTvL2eD8dVjQE9B3KTcI_y7bLU3Vxcb92fi7u7FP-7-gH7jFzV</recordid><startdate>20190331</startdate><enddate>20190331</enddate><creator>Huang, Xiaoxu</creator><creator>Li, Zheng</creator><creator>Zhang, Qiang</creator><creator>Wang, Weizhi</creator><creator>Li, Bowen</creator><creator>Wang, Lu</creator><creator>Xu, Zhipeng</creator><creator>Zeng, Ailiang</creator><creator>Zhang, Xing</creator><creator>Zhang, Xuan</creator><creator>He, Zhongyuan</creator><creator>Li, Qiang</creator><creator>Sun, Guangli</creator><creator>Wang, Sen</creator><creator>Li, Qing</creator><creator>Wang, Linjun</creator><creator>Zhang, Lu</creator><creator>Xu, Hao</creator><creator>Xu, Zekuan</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20190331</creationdate><title>Additional file 6: of Circular RNA AKT3 upregulates PIK3R1 to enhance cisplatin resistance in gastric cancer via miR-198 suppression</title><author>Huang, Xiaoxu ; 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BGC823CDDP cells were transfected with miR-198 mimic alone or cotransfected with the indicated vectors upon CDDP exposure (15 μM) for 48 h. The results are presented as the mean ± SEM. *P<0.05, **P<0.01, ***P<0.001. (TIF 1650 kb)</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.7929242</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biological Sciences not elsewhere classified Biophysics Cancer Chemical Sciences not elsewhere classified FOS: Biological sciences FOS: Clinical medicine Genetics Hematology Immunology Marine Biology Molecular Biology Virology |
| title | Additional file 6: of Circular RNA AKT3 upregulates PIK3R1 to enhance cisplatin resistance in gastric cancer via miR-198 suppression |
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