Additional file 6: of Circular RNA AKT3 upregulates PIK3R1 to enhance cisplatin resistance in gastric cancer via miR-198 suppression

Figure S4. a Predicted secondary structure of circAKT3 using the Vienna RNA package. b The expression of miR-198 was analyzed using RT-qPCR in tissues of cohort 1. c, d, e The relative luciferase activities were analyzed in 293T cells cotransfected with miR-198 mimics or miR-NC and luciferase reporter vectors containing the WT or Mut 3’UTR of CHRM3 (C), HIPK2 (D), and MAFB (E). f & g The relative luciferase activities were analyzed in SGC7901CDDP(f) and BGC823CDDP(g) cells cotransfected with miR-198 mimics or miR-NC and luciferase reporter vectors PIK3R1 3’UTR (WT) or PIK3R1 3’UTR (Mut). h & i The expression levels of PIK3R1 (h) and miR-198 (i) in SGC7901CDDP and BGC823CDDP cells after transfection of PIK3R1 plasmids or miR-198 mimics were detected by RT-qPCR. j The IC50 of miR-198 was analyzed by the CCK8 assay. BGC823CDDP cells were transfected with miR-198 mimic alone or cotransfected with the indicated vectors upon CDDP exposure (15 μM) for 48 h. The results are presented as the mean ± SEM. *P