Selective validation of microarray results by qRT-PCR and immunohistochemistry

Differential expression of transcripts identified by the microarray analyses was examined using qRT-PCR and immunohistochemistry. Additional colon tumors from five (M; nuclear β-catenin-positive) mice and four (S; nuclear β-catenin-negative) mice were harvested, and qRT-PCR was performed on nine genes that exhibited representative strong or subtle patterns in the microarray analyses. All nine patterns detected in the microarray set were validated by the qRT-PCR results. Alox12, Arachidonate 12-lipoxygenase; Casp6, Caspase 6; Matn2, Matrilin 2; Ptplb, Protein tyrosine phosphatase-like B; Sox21, SRY (sex determining region Y)-box 21; Spock2, Sparc/osteonectin, CWCV, and Kazal-like domains proteoglycan (testican) 2; Tesc, Tescalcin; Tpm2, Tropomysin 2; Wif1, WNT inhibitory factor; Stmn1, stathmin 1; Ptp4a2, phosphatase 4a2. In (a), *< 0.05 and **< 0.01.Copyright information:Taken from "Transcriptional recapitulation and subversion of embryonic colon development by mouse colon tumor models and human colon cancer"http://genomebiology.com/2007/8/7/R131Genome Biology 2007;8(7):R131-R131.Published online 5 Jul 2007PMCID:PMC2323222.