Functional and biochemical characterization of the 20S proteasome in a yeast temperature-sensitive mutant, -10

Ome, 1 mM EDTA-Tris, pH 8.5, 10% DMSO, and 10 μM peptidyl substrates (: Suc-LLVY-MCA for chymotrypsin-like activity; : Z-LLE-MCA for PGPH activity; : Boc-LRR-MCA for trypsin-like activity) and increasing concentrations of linolenic acid at 37°C for 1 h. The reactions were started, stopped as described in methods. Symbols represent the wild-type strain (○) and the mutant (●). Values are means ± SD of three independent experiments.Copyright information:Taken from "Functional and biochemical characterization of the 20S proteasome in a yeast temperature-sensitive mutant, "http://www.biomedcentral.com/1471-2091/9/20BMC Biochemistry 2008;9():20-20.Published online 21 Jul 2008PMCID:PMC2515314.