TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation. Supplemental Figures S1 and S2

Supplementary Figure 1 – Heatmap of CHIR-TGF-β interaction effects Complete unsupervised clustering heatmap showing genes with significant interaction between CHIR and TGF-β treatment with all genes annotated. Plots show selected genes representing each type of interaction. Supplementary Figure 2 – Effect of recombinant proteins of secreted factors on organoid size Quantification of organoids size measured at day 14, using same cultures as in Figure 6 D&E. (A) Kruskall Wallis with Dunn’s post test, n>623 organoids from N=3, (B) Kruskall Wallis with Dunn’s post test, n>428 organoids from N=4, (C) Kruskall Wallis with Dunn’s post test, n>280 organoids from N=3. *** p