Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo-4

Ized to alanines (A). Western blot analysis of total lysates, cytoplasmic and nuclear fractions of COS-7 cells mock transfected, transfected with wild type ICD (ICD-WT) or transfected with the mutated NLS (NLS-Mut) constructs (B). Blots were probed with the anti-ICD, an antibody to the cytoplasmic protein vinculin, or an antibody to the nuclear protein Oct-1. Western blot quantification and calculation of the nuclear-to-cytoplasmic ICD ratio (C). Immunofluorescence staining of transfected COS-7 cells (D-G) and quantification of nuclear localization by cell counting (H). Both western blot analysis and immunofluorescence show that the overexpressed wild-type ICD is localized in the nucleus, whereas the NLS-Mut construct is predominantly found in the cytoplasm.Copyright information:Taken from "Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo"http://www.biomedcentral.com/1471-213X/8/30BMC Developmental Biology 2008;8():30-30.Published online 25 Mar 2008PMCID:PMC2289808.