Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV-1 activity by increasing the virion density-10

Ate cultures (HLAC) from tonsil in principle as described in the legend to Fig. 1. Each experiment was performed in triplicate, and 2–4 independent experiments were performed (see Table 1). Shown are arithmetic means ± SD from one experiment. (C) HIV-1replication kinetics in monocyte-derived macrophages under conditions of continuous extract exposure. HIV-1stocks were exposed to the indicated concentrations of lemon balm extract for 1 h at 37°C. Primary macrophages were challenged overnight with the virus-extract suspension, washed the following day, and then cells were continuously cultured in medium containing the indicated concentrations of extract. HIV-1 replication was monitored at days 1, 7, 12, and 16 post infection by p24 ELISA. (D) Relative levels of HIV-1 replication at the endpoint (day 16 post infection) relative to untreated controls with the ICindicated. (E) Viability. In parallel, monocyte-derived macrophages were exposed to the identical extract concentrations over the 16 day-period and then analyzed in a standard MTT assay. Shown are arithmetic means ± standard deviations relative to untreated controls (set to 100%) from one donor.Copyright information:Taken from "Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV-1 activity by increasing the virion density"http://www.retrovirology.com/content/5/1/27Retrovirology 2008;5():27-27.Published online 20 Mar 2008PMCID:PMC2288616.