Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV-1 activity by increasing the virion density-9

peppermint, (C) lemon balm, or solvent (HO) for 1 h at 37°C. Subsequently, Sup-T1 cells were challenged overnight with the virus-extract suspension and washed the following day. HIV-1 replication was monitored by p24 ELISA and values determined from culture supernatants taken at day 5 post infection are shown. Lower panels (viability): In parallel, Sup-T1 cells were exposed to the identical extract concentrations overnight, washed, and analyzed for viability in a standard MTT viability assay. Each experiment was performed in triplicate, and 4–6 independent experiments were conducted (see also Table 1). Given are arithmetic means ± standard deviations (SD) from one experiment. The indicated ICand CCvalues were determined by using Prism software (GraphPad, San Diego, CA).Copyright information:Taken from "Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV-1 activity by increasing the virion density"http://www.retrovirology.com/content/5/1/27Retrovirology 2008;5():27-27.Published online 20 Mar 2008PMCID:PMC2288616.