Effects of high levels of glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells-1

or 10M IGF-1 were subjected to SDS-PAGE as described in Materials and Methods. The membranes were incubated with antibodies raised against the 3βHSD (), p450scc (), StAR () and p450 aromatase () proteins. Equal protein loading was verified by reprobing membranes with an anti-vinculin antibody. Results are representative of at least three independent experiments. Blots were quantified and the 3βHSD, p450scc, StAR and p450 aromatase to Vinculin ratios are shown. The results are expressed as means ± SE. Bars with different letters are significantly different (p < 0.05). The letter "a" indicates values which are not significantly different from control (without FSH or IGF-1 and glucose).Copyright information:Taken from "Effects of high levels of glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells"http://www.rbej.com/content/6/1/11Reproductive biology and endocrinology : RB&E 2008;6():11-11.Published online 19 Mar 2008PMCID:PMC2277425.