Effects of mutant Rac isoforms on metastatic properties

Copyright information:Taken from "Rac1 and Rac3 isoform activation is involved in the invasive and metastatic phenotype of human breast cancer cells"Breast Cancer Research 2005;7(6):R965-R974.Published online 30 Sep 2005PMCID:PMC1410764.Copyright © 2005 Baugher et al.; licensee BioMed Central Ltd. MDA-MB-435Br1 cells transiently transfected with vector alone, myc-Rac1(G12V), or myc-Rac3(G12V), were subjected to adhesion, haptotaxis, and invasion assays. MDA-MB-435α6HG6 cells transiently expressing vector alone, myc-Rac1(T17N), or myc-Rac3(T17N), were subjected to adhesion, and invasion assays. Cells were counted under 200× Magnification for adhesions assays, and 400× Magnification for haptotaxis and invasion assays. Y-axis represents the number of cells/field for at least 20 microscopic fields per variant. Bars represent standard error of the mean, and are representative of at least three separate experiments. An asterix indicates a statistically significant difference compared to the control, vector alone, as determined by a paired Student's -test (< 0.05).