Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy-3

Rease cell survival by preventing PTEN from binding to p53. Binding of HIV-1 Tat to p53 may result in reduced levels of both p53 by destabilization and PTEN by downregulation of PTEN expression. In vitro binding assay: Lysates containing p53-FLAG were incubated with BSA (control) or HIV-1 Tat protein and then mixed with lysate containing PTEN V5-tag. Proteins bound to p53 were immunoprecipitated using anti-FLAG immobilized antibody and analyzed for PTEN-V5 tag levels by Western blotting. Ratios of PTEN-V5 levels normalized by p53-FLAG levels are shown.Copyright information:Taken from "Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy"http://www.retrovirology.com/content/5/1/11Retrovirology 2008;5():11-11.Published online 31 Jan 2008PMCID:PMC2265748.