Additional file 3: of Hypoxia potentiates gemcitabine-induced stemness in pancreatic cancer cells through AKT/Notch1 signaling

Figure S3. AKT suppression attenuates gemcitabine-enhanced migratory and invasive abilities. Two pancreatic cancer cell lines were pretreated with 20 μM LY294002 for 2 h and then treated with gemcitabine. (a, b) The migratory ability of the cells was evaluated by the transwell migration assay, and the relative migratory ability was calculated by determining the number of cells migrating to the lower chamber under microscopic observation. (c, d) The transwell invasion assay was performed to measure the change in relative invasive ability. The graphs shown are from three independent experiments. Scale bar, 100 μm. **P