Additional file 2: of Endothelial damage, vascular bagging and remodeling of the microvascular bed in human microangiopathy with deep white matter lesions

Video of vascular bags and string vessels. The video was constructed from 116 images taken with the 20x objective at equidistant 0.7â Îźm steps throughout the section. Vessels are double-labeled for collagen IV (COLL4) and lectin (UEA-l) using (immuno)histochemistry. Vessel pathology is shown in the deep white matter lesion (DWML) of a case with SVD and a contralateral subacute ischemic cerebral infarct (Case 14). Vascular bags formed by collagenous membranes around vessels are attached to the vessel walls. A string vessel with a short constricted segment becomes discontinuous. Therefore, the latter types of vessel remnants were called ghost string vessels or briefly string ghosts. In addition, an empty collagenous tube without an endothelial layer is seen that may correspond to an early non-collapsed string vessel. Please also note the multiple COLL4-positive dots in the white matter parenchyma, which form vessel-like shaped rows in z-stack images. (AVI 16324 kb)