Additional file 6: of Cleavage of the extracellular domain of junctional adhesion molecule-A is associated with resistance to anti-HER2 therapies in breast cancer settings

JAM-A overexpression is insufficient to improve cell viability following short-term treatment with HER2-targeted drugs. MDA-MB-231 cells overexpressing full-length human JAM-A (JAM+) or a pcDNA3 empty vector control (EV) were treated for 3 days in serum-free medium with the indicated concentrations of trastuzumab (A) or lapatinib (B) and subjected to MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) viability assays. In parallel, the same cell lines were treated for 6 days in complete medium with the indicated concentrations of trastuzumab (C) or lapatinib (D) and subjected to MTT viability assays. JAM-A overexpression did not alter the drug sensitivity of cells under any of the short-term conditions tested. (E) Representative protein overexpression of JAM-A in transfected MDA-MB-231 cells. (TIF 145 kb)