Retinoic acid reduces human neuroblastoma cell migration and invasiveness: effects on DCX, LIS1, neurofilaments-68 and vimentin expression-1

Copyright information:Taken from "Retinoic acid reduces human neuroblastoma cell migration and invasiveness: effects on DCX, LIS1, neurofilaments-68 and vimentin expression"http://www.biomedcentral.com/1471-2407/8/30BMC Cancer 2008;8():30-30.Published online 29 Jan 2008PMCID:PMC2254429.s RA treatment (-). PC analysis (panels and ) shows morphological changes induced by RA. After 6 days RA treatment the DCXcells are few () in comparison to the control (), and they have the morphology of undifferentiated I-type cells. On the contrary is increased the number of VIMcells and the differentiated N-type VIMcells do not show immunoreactivity for DCX (). Bar graphs show the decrease in the number of DCXcells and the increase in the number of VIMcells after RA treatment. Bars represent mean ± standard error, N = 8, double star = p < 0.01. Neither in control nor in 6 days RA treated samples there are DCX/VIMcells immunopositive for both DCX and VIM, but as previously observed in control conditions (see fig.1), some DCX/VIMcells are present after RA treatment (see the cell marked with *, panel ). As in the controls (), NF-68 localizes in all RA treated SK-N-SH cells (). It is important to note that after the first 2 days of treatment it is possible to observe N-type cells (white arrows) that start to express VIM while DCX is disappearing .