Retinoic acid reduces human neuroblastoma cell migration and invasiveness: effects on DCX, LIS1, neurofilaments-68 and vimentin expression-0

Copyright information:Taken from "Retinoic acid reduces human neuroblastoma cell migration and invasiveness: effects on DCX, LIS1, neurofilaments-68 and vimentin expression"http://www.biomedcentral.com/1471-2407/8/30BMC Cancer 2008;8():30-30.Published online 29 Jan 2008PMCID:PMC2254429.nels and ) clearly shows the heterogeneity of the cell population. The triple IF for DCX (), LIS1 () and TUB () shows an immunoreactive signal for DCX only in the N-type and in some I-type cells. The anti-LIS1 antibody stains all the cell types. When co-expressed by the same cell, TUB and DCX colocalize both in the cell body and along the neuritic processes (see inset 1 of panel ). The triple IF for DCX (), NF-68 () and VIM () shows that none of the DCXcells is immunoreactive for VIM. Vimentin is present merely in the S-type and in some I-type DCXcells. Some other I-type cells do express neither DCX nor VIM (arrow in the inset 2 of panel ). The NF-68 protein is expressed by all the cell subtypes.