Identification of a novel exon within the human ZBP-89 (ZNF148) locus

Copyright information:Taken from "An isoform of ZBP-89 predisposes the colon to colitis"Nucleic Acids Research 2006;34(5):1342-1350.Published online 3 Mar 2006PMCID:PMC1390687.© The Author 2006. Published by Oxford University Press. All rights reserved () The human ZBP-89 (ZNF148) locus spans 142 kb (upper panel) and encompasses three untranslated (–) and six coding (–) exons. A potential alternative exon 4B (shaded box) was identified at a position 4.2 kb upstream of exon 5. Arrows indicate the locations of forward (F) and reverse (R) RT–PCR primers used for expression analysis of FL ZBP-89 and the exon 4B variant. Selected intron sizes (kb) are indicated. () RT–PCR analysis of full-length (FL) and variant (ΔN) ZBP-89 mRNA expression, using actin (Ac) as control. The cDNAs were generated with cultured cell lines using primers 4A-F/5-R (726 bp product); primers 4B-F/6-R (432 bp product) and (Ac), Actin control primers (400 bp product). Whole cell RNA was isolated from ColoDM2, CaCo 2, and HCT116 human colon cell lines as well as paired normal colon and colon adenocarcinoma tissue. Full-length message is expressed from promoter 1 (Pro 1) and variant (ΔN) ZBP-89 mRNA is expressed from promoter 2 (Pro 2), as described in .