Biological activities of the serum opacity factor targeted to the phage by a Gram-positive signal sequence

Copyright information:Taken from "Direct selection and phage display of a Gram-positive secretome"http://genomebiology.com/2007/8/12/R266Genome Biology 2007;8(12):R266-R266.Published online 13 Dec 2007PMCID:PMC2246268. The serum opacity activity of the pSOF22 phagemid particles displaying the SOF22. A total of 10phagemid particles were used per 200 μl assay. Binding of the SOF22-displaying phagemid particles to human fibronectin detected by phage ELISA. A total of 10phagemid particles were used per assay, each carried out in a well of a 96-well plate. Samples: pSOF22 PP/d3 and pSOF22 PP/wt, phagemid particles displaying the SOF from M22, generated using VCSM13d3 and VCSM13 helper phage, respectively; pDJ01 PP/wt, the vector phagemid particles, generated using the VCSM13 helper phage. BSA, TE, PBS, and BSA are buffer controls. Each data point is an average of three replicas; error bars represent standard deviation.