Is highly expressed in CD24Sca-133A10mouse mammary basal/myoepithelial and human breast myoepithelial cells

Copyright information:Taken from "Regulator of G-protein signalling 2 mRNA is differentially expressed in mammary epithelial subpopulations and over-expressed in the majority of breast cancers"http://breast-cancer-research.com/content/9/6/R85Breast Cancer Research : BCR 2007;9(6):R85-R85.Published online 8 Dec 2007PMCID:PMC2246188. Flow cytometric staining profile of mouse mammary cell preparations stained with anti-Sca-1 and anti-CD24 antibodies together with either a nonspecific rat IgG and anti-rat-FITC or 33A10 and anti-rat FITC. The nonspecific IgG and 33A10 staining profiles of the CD24Sca-1basal/myoepithelial cells (33A10), CD24Sca-1(Esr1) luminal cells (33A10) and CD24Sca-1(Esr1) luminal cells (33A10) [14] are indicated. Mean fold differences ± 95% confidence limits in RNA abundance for the gene in CD24Sca-1basal/myoepithelial, CD24Sca-1(Esr1) luminal epithelial and CD24Sca-1(Esr1) luminal epithelial mouse mammary cells (= 3 for all samples) [14]. The dotted lines indicate the 95% confidence limits of the comparator sample. All samples show a significant difference to the comparator (**< 0.01). The basal myoepithelial cells also have a significantly higher level of expression than either of the two luminal populations. Mean fold differences ± 95% confidence limits in expression levels for the gene in myoepithelial and luminal epithelial human breast cells compared with human breast fibroblasts (comparator). See Materials and methods for details of the samples. The dotted lines indicate the 95% confidence limits of the comparator sample. Both samples show a significant difference to the comparator (**< 0.01) and the myoepithelial cells have a significantly higher level of expression than the luminal cells.