Isolation and characterisation of mammary epithelial cell subpopulations

Copyright information:Taken from "Regulator of G-protein signalling 2 mRNA is differentially expressed in mammary epithelial subpopulations and over-expressed in the majority of breast cancers"http://breast-cancer-research.com/content/9/6/R85Breast Cancer Research : BCR 2007;9(6):R85-R85.Published online 8 Dec 2007PMCID:PMC2246188. Flow cytometric staining profiles (dead and CD45cells excluded) of anti-Sca-1 and 33A10 stained, freshly isolated mouse mammary cell preparations together with nonspecific IgG-stained control. Graphical space representation of principal component analysis of mammary fibroblasts, Sca-133A10, Sca-133A10and Sca-133A10cells. Mean fold differences ± 95% confidence limits in RNA abundance measured by quantitative real-time PCR for estrogen receptor () and prolactin receptor () transcripts in Sca-133A10(= 5 samples) and Sca-133A10(= 3 samples) mammary subpopulations compared with bulk mammary cell preparations depleted for CD45cells (comparator; = 3 samples). The dotted lines indicate the 95% confidence limits of the comparator sample. All samples show a significant difference to the comparator (**< 0.01) [35].