Role of the long cytoplasmic domain of the SIV Env glycoprotein in early and late stages of infection-3

Copyright information:Taken from "Role of the long cytoplasmic domain of the SIV Env glycoprotein in early and late stages of infection"http://www.retrovirology.com/content/4/1/94Retrovirology 2007;4():94-94.Published online 14 Dec 2007PMCID:PMC2242802.or truncated Env with similar titer; the amounts of input virus was determined based on the infectious index (IU/ng) as described in Methods. At 24 h after infection samples of nuclear DNA were tested for the presence of SIV DNA by real-time PCR in a TaqMan thermal cycler. Nuclear DNA samples corresponding to equal numbers of cells infected by SIV were analyzed in parallel. Fluorescence was recorded as a function of PCR amplification cycle. Quantitative SIV determinations were made by comparison with a standard curve produced by using serial dilution of plasmid DNA. The ratios of replication levels in dividing:non-dividing cells are shown.