Additional file 2: of A suppressive role of guanine nucleotide-binding protein subunit beta-4 inhibited by DNA methylation in the growth of anti-estrogen resistant breast cancer cells

Figure S2. Knockdown of GNB4 in TAMR-1 and 182R-6 cells using siRNA. 182R-6 and TAMR-1 cells grown to 80% confluency were transiently transfected with either 30Â nM GNB4 siRNA or 30Â nM negative control siRNA; at 72Â h after transfection, whole cellular lysates were prepared and subjected to Western blot analysis using antibody against GNB4. (PPTX 508 kb)