Effects of suramin and heparin on UV-B-induced CDPK activity, MBPK activity, and gene expression and accumulation of catharanthine in cell suspension cultures

Copyright information:Taken from "UV-B-induced signaling events leading to enhanced-production of catharanthine in cell suspension cultures"http://www.biomedcentral.com/1471-2229/7/61BMC Plant Biology 2007;7():61-61.Published online 7 Nov 2007PMCID:PMC2213653. Six-day-old cell suspension cultures were pre-treated with suramin (Sur) or heparin (Hep) at the indicated concentrations and were irradiated with UV-B for 5 min. As control one set of cells was irradiated with UV-B alone or left un-irradiated and the crude extracts from all cells were prepared at the indicated times and assayed for the phosphorylation of H IIIS () and MBP () under standard conditions as described in materials and methods. A second set of cells was similarly treated and the total RNA was isolated at the indicated times and analyzed for the transcript levels of and by RT-PCR (). The third set of cells were pretreated with the highest concentration of inhibitor previously used followed by 5 min of UV-B irradiation. After treatment, cells were collected after 48 h and catharanthine content was determined by HPLC (). These experiments were performed in triplicates and repeated at least twice. Error bars represent mean ± SD (n = 3).