Increase in local protein concentration by field-inversion gel electrophoresis-2

Copyright information:Taken from "Increase in local protein concentration by field-inversion gel electrophoresis"http://www.proteomesci.com/content/5/1/18Proteome Science 2007;5():18-18.Published online 26 Sep 2007PMCID:PMC2211458.ditions. Each gel represents 100 μg of rat liver lysate separated by isoelectric focusing (IEF) using a non-linear pH 3–10 IPG strip in the first dimension and a Criterion precast SDS-10–20% PAG in the second dimension at room temperature. Control denotes CFE and pulsed denotes FIGE with a a/r of 400/106 msec in the second-dimension separation. Gels were stained with Coomassie blue. Spots selected for LC-MS/MS analysis are denoted by numbers, and A denotes the internal calibrator (see also Table 3). The internal calibrator is the internal controls for equivalent sample loading in both control and pulsed conditions.