Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV-6

Orter and the indicated expression plasmids (Mock indicates empty expression vector pcDNA3). (a-e, g-l) Representative histograms of replicate analyses with the YFP-fluorescence plotted against the PI-fluorescence intensities. Q1 gated cells display increased YFP fluorescence, Q2 gated cells are YFP/PI double-positive cells, Q3 are YFP/PI negative cell populations and Q4 represent PI positive cells. (f, l) Quantitative analysis of Q1 gated events from all replicates (n = 3) given as number of YFP-positive per 10000 (f, HEK293) or 5000 (l, NIH-3T3) cells, respectively.Copyright information:Taken from "Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV"http://www.biomedcentral.com/1472-6750/8/55BMC Biotechnology 2008;8():55-55.Published online 13 Jul 2008PMCID:PMC2483975.