Additional file 1: of Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus

Preparation of hIgG1-Fc-9Arg. A. C-terminal 9Arg extension of human IgG1-Fc by PCR. Lane 1. hIgG1-Fc-9Arg PCR (annealing Tm:55, 850 bp), Mw. DNA ladder, lane 2. hIgG1-Fc-9Arg PCR (annealing Tm:52, 850 bp). Template for PCR was an Avastin heavy chain. B. DNA sequence analysis of hIgG1-Fc-9Arg express...

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Hauptverfasser:	Seungbin Cha, Lee, Sun, Kang, Sung, Hasan, Mohammad, Kim, Young, Sungpil Cho, Yong-Kyu Lee
Format:	Video
Sprache:	eng
Schlagworte:	Biochemistry Biological Sciences not elsewhere classified Biophysics Cancer Cell Biology Chemical Sciences not elsewhere classified FOS: Biological sciences FOS: Chemical sciences FOS: Clinical medicine FOS: Health sciences FOS: Physical sciences Genetics Immunology Infectious Diseases Microbiology Molecular Biology Pharmacology Physical Sciences not elsewhere classified Virology
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Zusammenfassung:	Preparation of hIgG1-Fc-9Arg. A. C-terminal 9Arg extension of human IgG1-Fc by PCR. Lane 1. hIgG1-Fc-9Arg PCR (annealing Tm:55, 850 bp), Mw. DNA ladder, lane 2. hIgG1-Fc-9Arg PCR (annealing Tm:52, 850 bp). Template for PCR was an Avastin heavy chain. B. DNA sequence analysis of hIgG1-Fc-9Arg expression vector. C. SDS-PAGE with reduced hIgG1-Fc-9Arg. Mw. protein ladder (10–245 kDa), lane1. 0.1 μg, lane 2. one μg, land 3. ten μg and PAGE with non-reduced hIgG1-Fc-9Arg (lane 1) and Mw (protein ladder (10–245 kDa)). (PPTX 314 kb)
DOI:	10.6084/m9.figshare.6874163