A new GFP-tagged line reveals unexpected Otx2 protein localization in retinal photoreceptors-1

Copyright information:Taken from "A new GFP-tagged line reveals unexpected Otx2 protein localization in retinal photoreceptors"http://www.biomedcentral.com/1471-213X/7/122BMC Developmental Biology 2007;7():122-122.Published online 2 Nov 2007PMCID:PMC2204009.rol E9.5 embryos. Otx2-GFP is seen by direct GFP fluorescence visualisation and , , and mRNAs are detected by whole-mount hybridization. For E9.5 animals, upper and lower panels show embryos with weak and strong abnormalities respectively. Anterior is leftward. Scale bar: 500 μm. Detection of Otx2, Otx2-GFP and Otx1 proteins by western blot analysis of nuclear extracts from tails and heads of and E9.5 embryos using an anti-Otx antibody. Normal structure of the retina in surviving adults. Comparison of bright field (BF left panels), GFP fluorescence (middle panels) and GFAP expression (right panels) in adult retinas of the indicated genotype. Scale bars : 50 μm.