S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells-5

Copyright information:Taken from "S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells"http://www.biomedcentral.com/1471-2407/7/205BMC Cancer 2007;7():205-205.Published online 6 Nov 2007PMCID:PMC2180183.monstrates a significant amount of psoriasin expression in the absence of tetracycline. With tetracycline, the expression of psoriasin transcription is effectively suppressed. GAPDH assesses equal loading. 100 μg of protein lysate were loaded on the gel. , Mammary epithelial TAC2 cells with tetracycline-inducible (Tet-Off) psoriasin expression (TAC2-tet EGFP2-psoriasin) demonstrate 15% higher viability in psoriasin-expressing cells (Tet-) than non-expressing (Tet+) when IFN-gamma (1000 u- 2000 u) is added at seeding (* p = 0.01, one-tailed paired t-test). Viability was measured by MTS assay on day 3 and the quotient IFN-gamma/control is demonstrated. The assay was performed in duplicate.