S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells-0

Copyright information:Taken from "S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells"http://www.biomedcentral.com/1471-2407/7/205BMC Cancer 2007;7():205-205.Published online 6 Nov 2007PMCID:PMC2180183.00 u or 1000 u of IFN-gamma. IFN-gamma treatment had no effect on the protein levels of pro-caspase-3. Calgranulin B and calgranulin A () are not influenced by the IFN-gamma treatment. Equal amounts of protein lysate were loaded on the gel. , Treatment with 75 μM HOfor one hour followed by culture for 48 h in regular medium induces the expression of calgranulin A in MCF10A cells. 100 μg of protein lysate were loaded on the gel. , MDA-MB-468 cells with the endogenous expression of psoriasin show the time-dependent repression of psoriasin expression when treated 24, 48 and 72 hours with 1000 u of IFN-gamma. Equal amounts of protein lysate were loaded on the gel. Probing with tubulin/GAPDH assesses the equal loading of the samples. C = cells cultured in monolayer.