Supplemental Figure 5. L. monocytogenes membrane protrusions are collapsed and/or contorted in SKOV3 and A549 cell lines depleted of CD147

SKOV3 and A549 cells were treated with control non-targeting (A) or CD147 targeting siRNA (B), infected with wild-type L. monocytogenes for 6 hours, fixed, then stained with mouse monoclonal CD147 targeting antibodies (green), DAPI (blue) to visualize bacteria (open arrowheads) and Alexa594-phalloidin (red) to visualize F-actin and membrane protrusions. CD147 is present at the plasma membrane of normal protrusions (solid arrowheads) extending from control siRNA treated cells (A). Membrane protrusions (solid arrowheads) are collapsed and/or contorted in CD147 depleted cells (B). Scale bars are 5 μm.