Quantification of ODN delivered to cells by nucleoporation

Copyright information:Taken from "2′-Deoxy-2′-fluoro-β--arabinonucleic acid (2′F-ANA) modified oligonucleotides (ON) effect highly efficient, and persistent, gene silencing"Nucleic Acids Research 2006;34(2):451-461.Published online 18 Jan 2006PMCID:PMC1342038.© The Author 2006. Published by Oxford University Press. All rights reserved () Slot blot of ON obtained from K562 cell lysate supernatants, and residual cell pellet 5 min, as well as 24, 72 and 96 h post nucleoporation. Upper rows are PS-DNA and PS-2′F-ANA–DNA (MOH10) antisense standards applied to membrane for quantification purposes. After transfer to the membrane, the blots were probed with a radiolabeled complementary sequence. Columns show material from untreated control cells (control), and cells nucleoporated with PS-DNA and PS-2′F-ANA–DNA chimera (MOH10). () Densitometry analysis of slot blot containing ON present in cell lysates, and remaining pellets, 5 min and 24, 72 and 96 h post nucleoporation of material. Control cells were nucleoporated in the absence of exogenous ON.