ATP binding inhibits CSB mediated ssDNA annealing

Copyright information:Taken from "Cockayne syndrome group B protein has novel strand annealing and exchange activities"Nucleic Acids Research 2006;34(1):295-304.Published online 12 Jan 2006PMCID:PMC1331990.© The Author 2006. Published by Oxford University Press. All rights reserved () Effect of ATP and ATPγS on ssDNA annealing, catalyzed by CSBwt, CSBE646Q, CSBT912/913V and CSBQ942E (5 nM each). ssDNA annealing was assayed as indicated in the legend to . Nucleotide was included at 4 mM. () Quantification of the results in (A). Values are the average of at least three independent experiments. SDs are indicated by error bars. () A silver stain of the recombinant CSBwt, CSBE646Q, CSBT912/913V and CSBQ942E proteins (100 ng each) used in strand annealing assay after separation by SDS–PAGE.