Verification of the new ChIP assay in yeast

Copyright information:Taken from "Fast chromatin immunoprecipitation assay"Nucleic Acids Research 2006;34(1):e2-e2.Published online 5 Jan 2006PMCID:PMC1325209.© The Author 2006. Published by Oxford University Press. All rights reserved The new and traditional ChIP methods were used to assess recruitment of the yeast Sir2p to and an adjacent genomic locus. () Diagram of the yeast locus (). Primers for PCR analysis were designed to the indicated regions. () Results of ChIP analysis with either antibodies to Sir2p or no antibodies (mock IP). IPs and DNA purification using either the new (blue) or traditional (purple) methods were done in parallel with equal amounts of yeast chromatin. Purified DNA samples were analyzed as above with real-time PCR. Results are expressed as signal ratios of anti-Sir2p IP to mock IP. PCR were done in triplicates, data represented as mean ± SD.