Nucleotide alteration within the substrate domain

Copyright information:Taken from "Characterization of a catalytically efficient acidic RNA-cleaving deoxyribozyme"Nucleic Acids Research 2006;33(22):7164-7175.Published online 3 Jan 2006PMCID:PMC1325019.© The Author 2005. Published by Oxford University Press. All rights reserved Different nucleotides from both the 5′ and 3′ ends of the substrate were altered (deletion or mutation) to identify the catalytically important nucleotides. H = equimolar mixture of A, C and T; D = equimolar mixture of A, G and T; and V = equimolar mixture of A, G and C. = Adenine ribonucleotide, = Guanine ribonucleotide, = Cytosine ribonucleotide and = Uracil ribonucleotide. Nucleotides shaded in grey are catalytically important.