Sequence analysis of terminal pools from both 1 min and 5 h selection

Copyright information:Taken from "Characterization of a catalytically efficient acidic RNA-cleaving deoxyribozyme"Nucleic Acids Research 2006;33(22):7164-7175.Published online 3 Jan 2006PMCID:PMC1325019.© The Author 2005. Published by Oxford University Press. All rights reserved The variation index (VI) complied for 1 min selection () and 5 h selection () upon sequencing ∼40 clones from each G4 population. The variation index was calculated by dividing the observed mutation rate at each position by 24% (the mutation rate of the starting library). The wild-type sequence is shown at the bottom of each chart (the last 22 nt at the 3′ constant region is omitted for clarity). Only nucleotides shaded in the grey zones are partially mutagenized. The underlined nucleotides are the nucleotides either absolutely conserved (VI = 0) or highly conserved (VI ≤ 0.25). The letter or letters placed at the top of the VI bars that are significantly larger than 1 (meaning these nucleotides were hyper-mutagenized) indicated the dominant nucleotides in the selected deoxyribozyme clones at the given positions. The short black bars beneath certain nucleotides in (B) denote the nucleotides that are absolutely conserved in both selections.