Reduced expression of Jak-1 and Tyk-2 proteins leads to interferon resistance in Hepatitis C virus replicon-1

Copyright information:Taken from "Reduced expression of Jak-1 and Tyk-2 proteins leads to interferon resistance in Hepatitis C virus replicon"http://www.virologyj.com/content/4/1/89Virology Journal 2007;4():89-89.Published online 18 Sep 2007PMCID:PMC2075494.eplicon cell lines (9/13, 5/15 and KR) with high ISRE promoter activation were treated with or without interferon alpha 2b (1000 IU/ml) for 4 weeks in a growth medium containing G-418 (1 mg/ml). The ability of replicon cells to develop G-418 resistant cell colonies was examined. Cell colonies were developed only in low ISRE replicons. Three individual colonies from each dish were picked up and nine stable cell lines were generated.