Chemotaxis of human breast cancer cells in a concentration gradient (0–50 ng/ml) of EGF

Copyright information:Taken from "A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods"http://www.biomedcentral.com/1472-6750/7/60BMC Biotechnology 2007;7():60-60.Published online 20 Sep 2007PMCID:PMC2071914. (A) First and last images of cells in the gradient region after 5 hours. (B) Directional plot of migration angle in 3–45 ng/ml EGF gradients, grouped in 10° intervals. (C) Cells inside the dotted lines (3–45 ng/ml EGF) were tracked and analyzed. The EGF gradient profile is visualized by FITC-dextran (10 kD) with a similar molecular weight to EGF. Scale bars are 20 μm.