Quantitative analysis of C

Copyright information:Taken from "Transcription regulation of the EcoRV restriction–modification system"Nucleic Acids Research 2005;33(21):6942-6951.Published online 6 Dec 2005PMCID:PMC1310966.© The Author 2005. Published by Oxford University Press. All rights reservedEcoRV interaction with RV DNA. () Binding of C.EcoRV to DNA harboring mutation in C-box1, () binding of C.EcoRVC to DNA harboring mutation in C-box2, () binding of C.EcoRV to DNA containing wt C-box1 and wt C-box2. Data shown correspond to the average of 2–3 independent gel mobility shift experiments. Fractions of free DNA and protein–DNA complexes at each protein concentration were calculated as a ratio of band intensity of the band of interest to the sum of intensities of all bands in the lane. Solid lines represent the best nonlinear regression fit of experimental data to an appropriate binding model, as described in the text. Nonlinear regression was performed using SCIENTIST (Micromath Scientific Software, Salt Lake City, UT).