A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis-2

Copyright information:Taken from "A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis"http://www.biomedcentral.com/1472-6750/7/49BMC Biotechnology 2007;7():49-49.Published online 16 Aug 2007PMCID:PMC2040145.rom lane 2 amplified by rolling circle DNA synthesis. : Nicked RC1-product (Nt. Alw I). : Cleaved RC1-product (Mly I). : Same as lane 4. : Circularized probe ((-)-strand). Note the apparently complete ligation and the absence of a background smear. : Circularized probe from lane 8 amplified by rolling circle DNA synthesis. : Nicked RC2-product (Nt. Alw I). Lane 11: Cleaved RC2-product (Mly I).: Size markers. Primers used: Pr SF-WT90 (+) and Pr SF-WT90 (-). The gel was stained with SYBR Gold.