Additional file 2: of A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies

Figure S1. Cp 43 decreased tau phosphorylation in rat primary cortical neurons. A. Quantitative analysis of cleaved active Caspase 3 positive neurons (7 DIV or 17 DIV) treated with or without 30 μM Cp 43 (72 h). > 800 neurons were counted for each experimental condition and bars represent mean percentages ± SEM (n = 4). B. Neurons (14 DIV) were incubated without or with Cp 43 (5, 10 or 30 μM, 72 h) and lysates immunoblotted with antibodies to detect Synapsin 1, Synaptophysin or BIII tubulin. C. Quantitative analysis of the effects of Cp 43 on synaptic protein expression levels normalised to BIII tubulin. Bars represent the average ratio ± SEM (n = 7). D. Neurons (7 DIV) were treated with or without Cp 43 for 6 h as indicated and lysates immunoblotted with antibodies to detect tau-pS262/S356 (12E8), tau-pS202/T205/S208 (AT8), total tau or BIII Tubulin. E. Quantitative analysis of the effects of increasing Cp 43 concentration on the levels of phosphorylated tau (12E8 and AT8 epitopes). Data are normalised to total tau and are expressed as mean percentages ± SEM of the controls (n = 5). ** p