Additional file 1: of Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer’s disease brain

Virtual slides and counting method. (a) Virtual slide obtained from hippocampal area section. The section is double labeled for SphK2 and Aβ. (b) Representative scale matrix designed on entorhinal cortex used for neurons and Aβ deposits counting. Boxes extend from pial surface to white matter. The n...

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Hauptverfasser: Dominguez, Gaëlle, Marie-Lise Maddelein, Pucelle, Mélanie, Nicaise, Yvan, Claude-Alain Maurage, Duyckaerts, Charles, Cuvillier, Olivier, Marie-Bernadette Delisle
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Sprache:eng
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Zusammenfassung:Virtual slides and counting method. (a) Virtual slide obtained from hippocampal area section. The section is double labeled for SphK2 and Aβ. (b) Representative scale matrix designed on entorhinal cortex used for neurons and Aβ deposits counting. Boxes extend from pial surface to white matter. The number of fields mainly depends on the thickness of the cortex. To compare results from different individuals, it was necessary to standardize the number of fields. Thus, after the counting step, the columns were standardized to 10 fields [18]. (TIFF 319 kb)
DOI:10.6084/m9.figshare.6084974