Experimental conditions were the same as in A except that the total Ca concentrations in the cell suspension were those indicated in panels B to D

Copyright information:Taken from "Effects of age-dependent membrane transport changes on the homeostasis of senescent human red blood cells"Blood 2007;110(4):1334-1342.Published online 15 Aug 2007PMCID:PMC1939906.© 2007 by The American Society of Hematology Ionophore addition to 5% Hct RBC suspensions when the [Ca] was higher than 100 μM was followed by rapid and extensive RBC lysis, unrelated to slow Pcat-mediated hydration, thus precluding investigation of Pcat at higher Ca levels. In panel A the medium contained 50 μM Na-EGTA and no added calcium in order to chelate contaminant [Ca] (less than 10 μM) and ensure [Ca] levels below Gardos channel activation.