The ordinate reports the percent of RBCs harvested from the top of the DEP oil (●, δ less than 1

Copyright information:Taken from "Effects of age-dependent membrane transport changes on the homeostasis of senescent human red blood cells"Blood 2007;110(4):1334-1342.Published online 15 Aug 2007PMCID:PMC1939906.© 2007 by The American Society of Hematology117) or from the pellet under the oil (○, δ more than 1.117) after secondary incubation in cold 0K medium. Time = 0 is the moment the Ca-loaded, ionophore-free RBCs were diluted into medium 90K at 37°C to activate Ca extrusion by the PMCA. The pattern in panel A was observed in 7 of the 9 experiments of this series.