SycO overproduction in the mutant strains Δand Δin the presence of calcium ions

Copyright information:Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"http://www.biomedcentral.com/1471-2180/7/67BMC Microbiology 2007;7():67-67.Published online 5 Jul 2007PMCID:PMC1933539. Overproduction of SycO was analyzed in parental strain WA-314 and the isogenic deletion mutants Δand Δcultured at 37°C in the presence of 5 mM CaCl. After 2 h 1 mM IPTG was added and cultivation was continued for another 2 h. Pellet fractions were analyzed by immunoblotting with sera raised aginst YopO, SycO and YopH.