Immunofluorescence staining of mouse samples for basal/myoepithelial and luminal cytokeratins

Copyright information:Taken from "Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors"http://genomebiology.com/2007/8/5/R76Genome Biology 2007;8(5):R76-R76.Published online 10 May 2007PMCID:PMC1929138. Wild-type (wt) mammary gland stained for Keratins 8/18 (red) and Keratin 5 (green) shows K8/18 expression in luminal epithelial cells and K5 expression in basal/myoepithelial cells. Mouse models that show luminal-like gene expression patterns stained with K8/18 (red) and K5 (green). Tumor samples that show basal-like, or mixed luminal and basal characteristics by gene expression, stained for K8/18 (red) and K5 (green). A subset of ;TgMMTV-Cre;53tumors showing nodules of K5/K8/18 double positive cells. A splindloid tumor stained for K8/18 (red) and smooth muscle actin (green).